The official website of Hinke Dekter (Msc)
Hinke Dekter
Résumé
Experience
2018 - present
Account Manager
Boom BV, Meppel The Netherlands.
Function details:
- Account manager
2010 - 2018
Laboratory Manager
Leiden Center for Bioscience,
University of Applied Sciences Leiden
Function details:
- Supervise research teams and interns
- Organize daily operations of laboratory
- Assist in all aspects of data collection (ISO-9001)
- Quality officer, skilled with working with a LIMS system
- Working according to ISO-17025 guidelines
- Lead researcher in independent project
- Teacher (University of Applied Sciences Leiden / Hogeschool Leiden)
Research projects:
Development of fast diagnostic tools in the field of (medical) Life Sciences, agro-culture and marinebiology
Top skills (in this function):
- Laboratory management
- Molecular Biology (PCR, RT-(q)PCT, Sequencing, DNA/RNA extraction etc)
- Microbiology
- Bio-physical methods (MALDI-TOF MS, Raman spectroscopy)
- Immunology (ELISA,Western-blot, Immunoprecipitation)
2005 - 2010
Researcher
Department of Clinical Chemistry
Leiden University Medical Center (LUMC)
Research:
Development and validation of an ELISA-BASED assay to measure the mTOR activity in renal allograft patients.
Techniques:
- Development and optimalization of ELISA based assay’s
- SDS-PAGE
- Western-Blot
- Cell culture (human cells)
- Isolation of cells from full blood
- FACS analysis
- Confocal laser scanning microscopy and conventional fluorescence microscopy
- Immunochemical localization
2004 - 2005
Researcher (internship)
Department of Orthopaedics
University Mecical Center Utrecht (UMCU)
Research
siRNA-mediated inhibition of matrix degrading enzymes in osteoarthritic chondrocytes.
Techniques
- SDS-PAGE
- Western-Blot
- Cell culture (human cells, animal cells, transfection etc)
- Isolation of chondrocytes from human cartilage samples
- Confocal laser scanning microscopy and conventional fluorescence microscopy
- siRNA synthese
- siRNA transfection
- Immunochemical localization
2003 - 2004
Researcher (internship)
Department Otolaryngology/Head and neck surgery, section Tumorbiology
VU University Medical Center Amsterdam
Research:
Head and neck carcinogenesis of two special patient populations; Fanconi anemia patients and young adults.
Techniques:
- Radio-isotopic techniques (p53 sequencer)
- DNA extraction and purification from histological coupes
- Micro-dissection
- Electrophoresis
- Labelling of DNA (radioactive en fluorescent)
- PCR
- Microsattelite analysis (ABIPRISM sequencer)
- P53 mutation-analysis
Researcher (internship)
2002 – 2003
Department applied tumor virology (ATV)
Krebsforschungszentrum (Heidelberg, Germany)
Research:
Characterization of CML66 an interaction partner of the HPV11-L2 protein.
Techniques:
- SDS-PAGE
- recombinant DNA techniques
- Western-Blot
- Cell culture (human cells, animal cells, transfection etc)
- RNA- en DNA isolation
- Electrophoresis
- Cloning
- Confocal laser scanning microscopy
1999-2000
Researcher (internship)
Department of pathology
St. Anthonius Hospital (Nieuwegein, NL)
Research:
Finding the best colouring techniques for cytological coupes. Furthermore a traineeship in histological techniques.
Techniques:
- Preparing histological coupes (paraffine, freeze and plastic)
- Preparing cytological coupes
- Colouring techniques histology and cytology